Daryl Johnston is a former professional fullback. He played college football at Syracuse University, where he was an All-Big East selection in 1987 and an All-American in 1988. He rushed for 1,830 yards and caught 46 passes during his collegiate career.

Johnston played for the NFL's Dallas Cowboys. Johnston was selected by the Cowboys in the second round (39th overall) of the 1989 NFL Draft. Johnston played in 149 consecutive games from 1989-1997. He also became one of the greatest special teams players in franchise history. He scored 22 career touchdowns and had more receptions than carries. His 294 receptions is the third highest number among Cowboys running backs (as of 2021. In 1993 Johnston had 50 receptions and averaged 7.4 yards per catch. He was a member of three Super Bowl winning teams. Due mainly to Johnston's contributions, the NFL created the fullback position in the Pro Bowl.

In 2003, Johnston joined the program Players Inc Radio when it moved to Fox Sports Radio. He was the General Manager of the San Antonio Commanders of the Alliance of American Football in 2019, and currently serves as Director of Player Personnel for the AFL's Dallas Renegades.